JRC GMO-Amplicons | EURL GMFF

Record ID:	OL372240.1
Database:	nt	Go to original record
Description:	Smilax china chloroplast, complete genome.
Taxon:	Smilax china
Original size:	157959 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46610	47226	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgatta gcttgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aattcaaaat
101   gggcaatcct gagccaaatc tttattttga taaaataggg gtttaattta
151   taaaactaga atcaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgacta cgttacgttg cgttggtagc tggaatccct
251   ctatcaaaat tacagatagg atggccctat atacgtatat atacatactg
301   acatatcaaa cgattgatta attatgaata tatttatata tatatatata
351   tgatatgaat attatgatat gaatatgaaa aaaaaaaatt tagagttatt
401   gtgaatctat tccaatcgaa gttgaagaaa gaatcgaata ttcagttatc
451   aaattattca ttccagagtc tgaaaaatct tttgaaaaac tgattaattg
501   gacgagaata aagagagagt cccattctac atgtcaatat cgacaacaat
551   gaaatttata gtaaaaggaa aatccgtcga ctttagaagt cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details