JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ902354.1
Database:	nt	Go to original record
Description:	Impatiens balsamina chloroplast, complete genome.
Taxon:	Impatiens balsamina
Original size:	152518 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46379	46974	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatggacaat cctgagccaa atccggtttt tggaaaacaa atccaaataa
151   agattccgaa accaaaaata gagataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaattcg tggatcgaaa
251   ctttagaaag tatgaaagaa aaacatatat atatatatgc ataagtatat
301   atgcataaga aaatgaatga cgatccaaaa gcgtatccgt ttttttagga
351   aaaaaatggt tgtgaatcga ttccatattc aataaagaat caaatattca
401   ttggttcaaa tattcattgg ttaaattatt catttcatac tccatagtct
451   gatagttcct ctgaagaact gattaatcag acgagaataa agatagagtc
501   ccattataca tgtcaatcct gacaacaatg aaatttatag taataggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details