JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641375.1
Database:	nt	Go to original record
Description:	Phlomis fruticosa isolate BPTPS185 voucher LISE:96495 chloroplast, complete genome.
Taxon:	Phlomis fruticosa
Original size:	151073 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45962	46524	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttt ttaaacaaag
151   gttcaaaaaa cgaaataaag gataggtgca gagactcaat ggaagctgtt
201   ctaacaaatg gagttgactg cgttggtaga ggaatccttt ctacggaaac
251   ttcagaaagg atgaaggata aacgtatcta tcgaatactt tatcaaatga
301   ttaatgatgg accgaatccg tatttttaat atgaaaaata gaagaattgg
351   tgtgaattga ttctatattg aagaaaaaat cgaatattca ttcatcaaat
401   cattcactcc atagttcgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caatactggc aacaatgaaa
501   tttatagtaa gaggaaaatc cgtcgacttt taaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details