JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641368.1
Database:	nt	Go to original record
Description:	Aloysia citrodora isolate BPTPS172 voucher LISE:96483 chloroplast, complete genome.
Taxon:	Aloysia citrodora
Original size:	155230 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48436	48997	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgccaaag ggggataggt gcagagactc aatggaagct gttctaacaa
201   acggagttga ctgcgtgggt agaggaatct ttccatcgaa acttcagaaa
251   ggatgaagga taaacgtatc tattgaatac tctatcaaat gattaatgat
301   ggcccgaatc tgtatctgta tttttttata tgaaaaatgg aagaattggt
351   gtgaattgat tccacattga agaaagaatc aaatagtcct tcatcaaatc
401   attcactcca tagtccgata aatcttttaa agaacggatt aatcgtacga
451   gaataaagat agagtcccat tctacatgtc aataccggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details