JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641359.1
Database:	nt	Go to original record
Description:	Helichrysum italicum subsp. picardii isolate BPTPS157 voucher LISE:96468 chloroplast, complete genome.
Taxon:	Helichrysum italicum subsp. picardii
Original size:	152112 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46921	47472	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgccgg tagaggaatc tttccatgga aactttagaa
251   aggataaacg catctattga atactatatc aaatgattaa tgttggcccg
301   aatctgtttt tttaatatga aaatgaaaaa atggaaaaat cggtgtgaat
351   ttatttcacg ttgaagaata aatcgaatat tcatcaactc attcactcca
401   tagtccgata gatcttttaa agaacttatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details