JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641292.1
Database:	nt	Go to original record
Description:	Origanum vulgare subsp. virens isolate BPTPS018 voucher LISE:96353 chloroplast, complete genome.
Taxon:	Origanum vulgare subsp. virens
Original size:	151826 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46857	47400	+	544 bp

Amplicon:

544 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggataggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tgcgtcggta
201   gaggaatctt tccatggaaa ctttctaaag gatgaaagat aaacgcatct
251   attgaatact atatcaaatg attaatgttg gcccgaatct atttttttaa
301   tatgaaaatg aaaaaatgga aaaatcggtg tgaatttatt tcacgttgaa
351   taaaaaatag aatattcatc aacttattaa ctccatagtc cgatagatct
401   tttaaagaac tttttaatcg gacgagaata aagatagagt cccattctac
451   gtgctacata tcaataccgg caacaatgaa atttatagta agaggaaaat
501   ccgtcgactt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	544	525	2.45e-08

Match details