JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684560.1
Database:	nt	Go to original record
Description:	Mentha cervina isolate BPTPS179 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Mentha cervina
Original size:	590 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	589	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtc ggtagaggaa tctttccatg gaaactttat
251   tttataaagg atgaaagata aacgcatcta ttgaatacta taatcaaatg
301   attaatgttg gcccgaatct atttttttaa tatgaaaatg aaaaaatgga
351   aaaatcggtg tgaatttttt tcacgttgaa gaaaaaatag aatagaatat
401   tcatcaactc attcactccg tagtccgata gatcttttaa agaacttatt
451   aatcggacga gaataaagat agagtcccat tctacatgct acatatcaat
501   accggcaaca atgaaattta tagtaagagg aaaatccgtc gactttaaaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details