JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684553.1
Database:	nt	Go to original record
Description:	Mentha cervina isolate BPTPS154 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Mentha cervina
Original size:	591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	590	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttgactgcgt cggtagagga atctttccat ggaaacttta
251   ttttataaag gatgaaagat aaacgcatct attgaatact ataatcaaat
301   gattaatgtt ggcccgaatc tattttttta atatgaaaat gaaaaaatgg
351   aaaaatcggt gtgaattttt ttcacgttga agaaaaaata gaatagaata
401   ttcatcaact cattcactcc gtagtccgat agatctttta aagaacttat
451   taatcggacg agaataaaga tagagtccca ttctacatgc tacatatcaa
501   taccggcaac aatgaaattt atagtaagag gaaaatccgt cgactttaaa
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details