JRC GMO-Amplicons | EURL GMFF

Record ID:	ON684546.1
Database:	nt	Go to original record
Description:	Salicornia fruticosa isolate BPTPS109 tRNA-Leu (trnL) gene, complete sequence; chloroplast.
Taxon:	Salicornia fruticosa
Original size:	608 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	607	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaatta gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaaa
101   aaagggcaat cctgagccaa ctcctttttt caaaataaaa aaaggatagg
151   tgcagagact caaaggaagt tgttctaaca aatgggtttg ggttgactgt
201   gttgttctaa gaattcttcc gtcaaaactc caatctaaaa agggtgaaga
251   atatactatg tcaaatgatt aatgacaccc cgaatctatt ttgtattttt
301   ttttctaatt ttttagtatt tattatttct atatttaata cagaagatga
351   aattctcgct aaataaaaat tgcgaatatg aaatgaaaat attatatgaa
401   taggaaaaaa agggaagaat ttttgtgaat cgatttcaaa ttgaaaaaga
451   atcaaatatt cattcatcaa atcattcact cccgagaata aagatagagt
501   cccattctac atgtcaatac tgacaagaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details