JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ636547.1
Database:	nt	Go to original record
Description:	Prunella vulgaris chloroplast, complete genome.
Taxon:	Prunella vulgaris
Original size:	151514 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46723	47296	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa agggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggaatct ttccatggaa actttagaaa
251   ggataaacgc atctattgaa tactatatca aaatcaaatg attaatgttg
301   gcccaaatct gttttgtttt tttaatataa aaatgaaaaa atggaaaaat
351   cggtgtgaat ttttttcacg ttgaagaaaa aatcaaatat tcatcaactc
401   attaactcca tagtccgata gatcttttaa agaacttatt aatcggacga
451   gaataaagat agagtcccat tctacatgct acatatcaat tcaataccgg
501   caacaatgca atttatagta agaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details