JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ636545.1
Database:	nt	Go to original record
Description:	Dianthus chinensis chloroplast, complete genome.
Taxon:	Dianthus chinensis
Original size:	149640 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46635	47251	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tattataaga cttcttccgt ctaaattccc ataaaaaggg
251   ggggtaaaat cgacggagtg aaatgattaa tgacaacccg aatctgttct
301   gtattttttt atatatatta tatatataaa tccatacttt attctatatt
351   tagagtagag atctagaaga ttaaatgaaa aaatgcaaga attcttgtga
401   atccattcaa agttgaaagc agaatcaatc aagatttatt cattaaaata
451   ttcacactaa ctccatagtc tgatagatct tgtgaagaac ttattaatcg
501   gatgagaata aagatagagt cccgtactac atgtcaatac tgacaacaat
551   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat catgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details