JRC GMO-Amplicons | EURL GMFF

Record ID:	MT526777.1
Database:	nt	Go to original record
Description:	Amaranthus blitum chloroplast, complete genome.
Taxon:	Amaranthus blitum
Original size:	150596 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47458	48075	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaaaaaat
151   acggattcag aaagcaagaa taaaaaaaag gataggtgca gagactcaaa
201   ggaagctgtt ctaacaaatg gagttgattg tgctgggttg ttcgaagaat
251   tcttccgtcg aaattcctga ttaatggcac ttcgaatcta ttctgtattt
301   ctatttttat aatttttaga tatttctgat ttcgagataa tatagaatat
351   gaaattttct agaaataaaa atgaatatga aaaaatggaa gaattttttt
401   gactctattt caagttgaaa aaagaatcaa atattcatca aatcattcac
451   tccatagtct gatagatctt tctttgaaag aaccggttaa tcggttactc
501   ggacgagaat aaagatagag tcccattata catgtcaata ctgacaacaa
551   tgaaatttat agtacgagga aaatccgtcg actttaaaaa tcgtgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details