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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: OM397417.1
Database: nt Go to original record
Description: Synthetic construct 5-enolpyruvylshikimate 3-phosphate (epsps) gene, complete cds.
Taxon: synthetic construct
Original size: 2075 bp

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Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target:CaMV P-35S

View other 10854 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 29 222 + 194 bp
Amplicon: 194 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcagatg
51    cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
101   gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
151   tgacatctcc actgacgtaa gggatgacgc acaatcccac tatc

View other 1 match with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.08e-08
primerB 100.0 20 bp 0 0 194 175 9.23e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.