JRC GMO-Amplicons | EURL GMFF

Record ID:	MT150107.1
Database:	nt	Go to original record
Description:	Dianthus juniperinus subsp. aciphyllus plastid, complete genome.
Taxon:	Dianthus juniperinus subsp. aciphyllus
Original size:	149607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46583	47201	+	619 bp

Amplicon:

619 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaaag
251   ggggggtaaa atcgacggag tgaaatgatt aatgacaacc cgaatctgtt
301   ctgtattttt tttttttttt tttatatata aatccatact ttattctata
351   tttagagtag agatctagaa gattaaatga aaaaatgcaa gaattcttgt
401   gaatccattc aaagttgaaa gcagaatcaa tcaagattta ttcattaaaa
451   tattcacact aactccatag tctgatagat cttgtgaaga acttattaat
501   cggatgagaa taaagataga gtcccgtact acatgtcaat actgacagca
551   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcatgaggg
601   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	619	600	2.79e-08

Match details