JRC GMO-Amplicons | EURL GMFF

Record ID:	MT150105.1
Database:	nt	Go to original record
Description:	Dianthus fruticosus subsp. fruticosus plastid, complete genome.
Taxon:	Dianthus fruticosus subsp. fruticosus
Original size:	149253 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46572	47189	+	618 bp

Amplicon:

618 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaagg
251   gggggtaaaa tcgacggagt gaaatgatta atgacaaccc gaatctgttc
301   tgtatttttt tttatatatt atatatataa atccatactt tattctatat
351   ttagagtaga gatctagaag attaaatgaa aaaatgcaag aattcttgtg
401   aatccattca aagttgaaag cagaatcaat caagatttat tcattaaaat
451   attcacacta actccatagt ctgatagatc ttgtgaagaa cttattaatc
501   ggatgagaat aaagatagag tcccgtacta catgtcaata ctgacaacaa
551   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcatgagggt
601   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	618	599	2.78e-08

Match details