JRC GMO-Amplicons | EURL GMFF

Record ID:	MT150102.1
Database:	nt	Go to original record
Description:	Dianthus fruticosus subsp. amorginus plastid, complete genome.
Taxon:	Dianthus fruticosus subsp. amorginus
Original size:	149623 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46579	47192	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aataaaaaaa
101   agggtaatcc tgagccaact ccttttgttt ccttttttca aaagaaaaaa
151   aggataggtg cagagactca aaggaagctg ttctaacaaa ttgggttgac
201   tgtgctgtgt tgttataaga cttcttccgt ctaaattccc ataaaaaagg
251   gggggtaaaa tcgacggagt gaaatgatta atgacaaccc gaatctgttc
301   tgtatttttt tttatatatt atataaatcc atactttatt ctatatttag
351   agtagagatc tagaagatta aatgaaaaaa tgcaagaatt cttgtgaatc
401   cattcaaagt tgaaagcaga atcaatcaag atttattcat taaaatattc
451   acactaactc catagtctga tagatcttgt gaagaactta ttaatcggat
501   gagaataaag atagagtccc gtactacatg tcaatactga caacaatgaa
551   atttatagta agaggaaaat ccgtcgactt taaaaatcat gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details