JRC GMO-Amplicons | EURL GMFF

Record ID:	LN684311.1
Database:	nt	Go to original record
Description:	Cucumis melo genomic scaffold, unassembled_sequence31859
Taxon:	Cucumis melo
Original size:	1469 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	265	877	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttttttccg taaaaaaaaa agggtaggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac gacgttgcgt
201   tagtaaagga atgaatcctt ccatcgaaac tccagaaacg atgaaagata
251   aacgtattac gtactgaaat actatattga ttaatgacaa ctcgaatctc
301   taaattttta tatatttcta taattttttt ttatatttat atatatatat
351   aaaattatat ataaaaatga acgaattgtt atgaatcgat tccaagatct
401   acaagttgta aaaaaaaaat aatcgaatat tcattgatca aatcatttac
451   tccatcatag tctgatagat cttttgaaga actgattaat cagatcgaat
501   agaataaaga tagagtccca ttctacatgt caataacgac aaaaatgaaa
551   tttatagtaa gaggaaaatc cgtcgacttt taaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details