JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ136952.1
Database:	nt	Go to original record
Description:	Casasia clusiifolia voucher FG 67-859 tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Casasia clusiifolia
Original size:	1731 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	797	1367	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaaaaagggg caatcctgag ccaaatcccg ttttccgaaa ccaaaggttc
151   agaaagtgaa aaagggatag gtgcagagac tcaacggaag ctgttctaac
201   aaatggagtt ggctgcgtta gtagagaaat ctttccatct aaaattccga
251   aaggataaag tgaaggataa acgtatatat acgtattgaa tactatatca
301   aatgattaat gacgactcga ctgaatctgt attttttata tgaaaaggga
351   agaattggtg tgaatagatt ccacattgaa gaaggaatcg aatattcatt
401   gatcaaatga ttcactccat agtctgatag atcttttcaa gaattgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca atgtcggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details