JRC GMO-Amplicons | EURL GMFF

Record ID:	ON212126.1
Database:	nt	Go to original record
Description:	Lonicera caerulea voucher EE_3 GMC tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Lonicera caerulea
Original size:	1015 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	586	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    actactaagt gagaactttc aaattcagag aaaccccgga attaataaaa
101   atgggcaatc ctgagccaaa tccagttttc cgaaaacaag ggtttagaaa
151   gcaaaaatca aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaacggagtt gactgtcgtg tgttggtaga aagaatcctt ccatagaaac
251   ttcagaaagg ataaacctat aaacatagat atacgtattg aaatgctatg
301   atactatatc aaatgattaa tgacgatcct aatttgtatc tgtattttat
351   atatatcaaa atggaagaat tgttgtgaag tgattccata ttgaagaaag
401   aatcaaatat tcattgatca aatcattcac tccatagtct gatcgatctt
451   ttgaagaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaagacc ggcaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttagaaaty gtgagggttc aagtccctct atcccc

View other 61 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details