JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ428185.1
Database:	nt	Go to original record
Description:	Trichosanthes villosa isolate PEBO4037 PsbD (psbD) and PsbC (psbC) genes, complete cds; tRNA-Ser (trnS-UGA) gene, complete sequence; PsbZ (psbZ) gene, complete cds; tRNA-Gly (trnG-GCC) and tRNA-Met (trnM-CAU) genes, complete sequence; ribosomal protein S14 (rps14), PsaB (psaB), PsaA (psaA), and Ycf3 (ycf3) genes, complete cds; tRNA-Ser (trnS) gene, complete sequence; ribosomal protein S4 (rps4) gene, complete cds; tRNA-Thr (trnT), tRNA-Leu (trnL-UAA), and tRNA-Phe (trnF-GAA) genes, complete sequence; and NdhJ (ndhJ), NdhK (ndhK), and NdhC (ndhC) genes, complete cds; chloroplast.
Taxon:	Trichosanthes villosa
Original size:	19883 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	15739	16331	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa ggggggtagg
151   tgcagagact caatggaagc tgttctaaca aatggagttg actacattgc
201   gttagtaaag gaatgaatcc ttctatcgaa actccagaaa agatgaaaga
251   taaacgtatt acgtactgaa atactatttg attaatgaca acccgaatct
301   ctattttttt ttttttttat ataaatataa aaaatgatat ataaaaatga
351   acgaattgtt ctgaatcgat tccaatatct ccaagttgaa aaaagaatcg
401   aatattcatt gatcaaatca tttactccat catacatagt ctgatagatc
451   ttttgaagaa ctgattaatc agatgcgaat agaataaaga tagagtccca
501   ttctacatgt caataccgac aaaaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details