JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ428161.1
Database:	nt	Go to original record
Description:	Trichosanthes sericeifolia isolate HLQW090094 01 tRNA-Leu (trnL-UAA) and tRNA-Phe (trnF-GAA) genes, complete sequence; chloroplast.
Taxon:	Trichosanthes sericeifolia
Original size:	1701 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	553	1138	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaag ggggtaggtg
151   cagagactca atggaagctg ttctaacaaa tggagttgac tacattgcgt
201   tagtaaagga atgaatcctt ctatcgaaac tccagaaaag atgaaagata
251   aacgtattac gtactgaaat actatttgat taatgacaac ccgaatctcg
301   attttttttt tatataaata taaaaaatga tatataaaaa tgaacgaatt
351   gttctgaatc gattccaata tctccaagtt gaaaaaagaa tcgaatattc
401   attgatcaaa tcatttactc catcatacat agtctgatag atcttttgaa
451   gaactgatta atcagatgcg aatagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaaaaatg aaatttatag taagaggaaa atccgtcgac
551   tttaaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details