JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ428135.1
Database:	nt	Go to original record
Description:	Trichosanthes pilosa isolate HLQW090073 02 tRNA-Leu (trnL-UAA) and tRNA-Phe (trnF-GAA) genes, complete sequence; chloroplast.
Taxon:	Trichosanthes pilosa
Original size:	2183 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	616	1202	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaggg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcggtag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaaccca aatctctatt
301   tttttttttt ttatataaat atcaaaaatg atatataaaa atgaacgaag
351   tgttctgaat cgattccaat atctccaagt tgaaaaaaga atcgaatatt
401   cattgatcaa atcatttact ccatcataca tagtctgata gatcttttga
451   agaactgatt aatcagatgc gaatagaata aagatagagt cccattctac
501   atgtcaatac cgacaaaaat gaaatttata gtaagaggaa aattcatcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details