JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ428089.1
Database:	nt	Go to original record
Description:	Trichosanthes parviflora isolate PE6072 tRNA-Leu (trnL-UAA) and tRNA-Phe (trnF-GAA) genes, complete sequence; and NdhJ (ndhJ) gene, complete cds; chloroplast.
Taxon:	Trichosanthes parviflora
Original size:	2877 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	633	1215	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaagg gtaggtgcag
151   agactcaatg gaagctgttc taacaaatgg agttgactac gttgcgttag
201   taaaggaatg aatccttcta tcgaaactcc agaaaagatg aaagataaac
251   gtattacgta ctgaaatact atttgattaa tgacaacccg aatctctatt
301   ttttttttat ataaaaataa aaaatgatat ataaaaatga acgaattgtt
351   ctgaatcgat tccaatatct ccaagttgaa aaaagaatcg aatattcatt
401   gatcaaatca tttactccat catacatagt ctgatagatc ttttgaagaa
451   ctgattaatc agatgcgaat agaataaaga tagagtccca ttctacatgt
501   caataccgac aaaaatgaaa tttatagtaa gaggaaaatc cgtcgacttt
551   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details