JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ427974.1
Database:	nt	Go to original record
Description:	Trichosanthes kerrii isolate PT6926 tRNA-Leu (trnL-UAA) and tRNA-Phe (trnF-GAA) genes, complete sequence; NdhJ (ndhJ), NdhK (ndhK), and NdhC (ndhC) genes, complete cds; tRNA-Val (trnV), tRNA-Thr (trnT-GGU), and tRNA-Met (trnM-CAU) genes, complete sequence; and AtpE (atpE), AtpB (atpB), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL), AccD (accD), PsaI (psaI), Ycf4 (ycf4), CemA (cemA), PetA (petA), PsbJ (psbJ), PsbL (psbL), PsbF (psbF), and PsbE (psbE) genes, complete cds; chloroplast.
Taxon:	Trichosanthes kerrii
Original size:	18364 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	559	1148	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaaaaaaaaa gggggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacattgcg
201   ttagtaaagg aatgaatcct tctatcgaaa ctccagaaaa gatgaaagat
251   aaacgtatta cgtactgaaa tactatttga ttaatgacaa cccgaatctc
301   tattttattt ctgttattta aatataaaaa atgatatata aaaatgaacg
351   aattgttctg aatcgattcc aatatctcca agttgaaaaa agaatcgaat
401   attcattgat caaatcattt actccatcat acatagtctg atagatcttt
451   tgaagaactg attaatcaga tgcgaataga ataaagatag agtcccattc
501   tacatgtcaa taccgacaaa aatgaaattt atagtaagag gaaaatccgt
551   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details