JRC GMO-Amplicons | EURL GMFF

Record ID:	OM044392.1
Database:	nt	Go to original record
Description:	Bougainvillea stipitata var. grisebachiana voucher SZG:2015061601 chloroplast, complete genome.
Taxon:	Bougainvillea stipitata var. grisebachiana
Original size:	154679 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48473	49089	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgcaaatttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa ctcctttttt cttttcaaaa gaaaaggttc
151   agaaagcaag aaaaaaagga taggtgcaga gactcaaagg aagctgttct
201   aacaaatggg gttgactgtg ctgtgttgtt atcagaattc ttccatcaaa
251   attccaatcc aaaaaaaaaa gggtgaagca tatacgtact caactatatc
301   aaataattaa taacaacctg aatcggtatt ttttttttct catttttata
351   tatttatgaa atgaaaaaat ttatatgaga aaaaaatgga agaattcttg
401   tgattgtgaa tcgattccaa gttgaaaaaa gaatcaaata ttcattcatc
451   aaaccattca ctccatagtc tgatagattt tttgaagaac tgattaatcg
501   gacgagaatg aagagagagt ccagttctac atgtcaatac tgacaacaat
551   gaaatttaga ataagaagaa aatccgtcga ctttaaaaat cgtgagggtt
601   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details