JRC GMO-Amplicons | EURL GMFF

Record ID:	OM174293.1
Database:	nt	Go to original record
Description:	Papaver rhoeas chloroplast, complete genome.
Taxon:	Papaver rhoeas
Original size:	152948 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46414	47015	+	602 bp

Amplicon:

602 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaatta agggatttct
151   gaaagcgaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga gtagaggact ccttctattc
251   aatttgaatt taaactccat aaagaatgag ggataaacct acgatacgta
301   cgtatacgta ctgaaatatc aaagattcta ttaatattaa tgtcgcccta
351   aaattttttt tacataaaaa aaggaataat ttttgtgaat ctattccaag
401   ttgaaggaag aatcgaatat tcagtaatca aaccattcac tccatagtct
451   gatatatctt ttgacgaact gattaatcgg acgagaataa agatagagtc
501   ccgttctaca tgtcaatatc aatacagaca acaatgaaat ttatagtaag
551   aggaaaatcc gtcgacttta taaatcgtga gggttcaagt ccctctatcc
601   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	602	583	2.71e-08

Match details