JRC GMO-Amplicons | EURL GMFF

Record ID:	OM174283.1
Database:	nt	Go to original record
Description:	Papaver hultenii var. salmonicolor chloroplast, complete genome.
Taxon:	Papaver hultenii var. salmonicolor
Original size:	153668 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46595	47190	+	596 bp

Amplicon:

596 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaatt aagggatttc
151   tgaaagcgag aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaatgaat ggagttgact gcgttggtag aggaatcctt ctattaaatt
251   tgaatttaaa ctccataaag aatgagggat gaacctatat acgatacgta
301   cgtatacgta ctgaaatatc aaagattcta ttaatgtcgc cctaactatt
351   tttttacata aaaaaagaaa taatttttgt gaatttattc caagttgaag
401   gaagaatcga aaattcagtg atcaaaccat tcactccata gtctgataga
451   tcttttgatg aactgattaa tcggacgaga ataaagatag agtcccgttc
501   tacatgtcaa tatcaatacc gacaacaatg aaatttatag taagaggaaa
551   atccgtcgac tttataaatc gtgagggttc aagtccctct atcccc

View other 14 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	596	577	2.68e-08

Match details