JRC GMO-Amplicons | EURL GMFF

Record ID:	ON597627.1
Database:	nt	Go to original record
Description:	Citrullus rehmii chloroplast, complete genome.
Taxon:	Citrullus rehmii
Original size:	157071 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49706	50292	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aagggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatcct tccatcgaaa ctccagaaaa gatgaaagat
251   aaacgtatta cgtactgaaa tactatattg attaatgaca actcgaatct
301   ctattttttt atatttatat ataaaaaatg atatataaaa atgaacgaat
351   tgttatgaat cgattccaat atctccaagt tgaaaaaaga atcgaatatt
401   cattgatcaa atcatttact ccatcataca tagtctgata gatcttttga
451   agaacttatt aatcagatgc gaatagaata aagatagagt cccattctac
501   atgtcaatac cgacaaaaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details