JRC GMO-Amplicons | EURL GMFF

Record ID:	OK649927.2
Database:	nt	Go to original record
Description:	Nyctocalos pinnatum chloroplast, complete genome.
Taxon:	Nyctocalos pinnatum
Original size:	159591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48451	49014	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttagttg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt ggctgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgaag gataaacgta tctattgaat actatatcaa aggcttaatg
301   atggcccgaa tctgtatctg tattttttta tatgaaaaat ggaagaatta
351   gtgtgaattg attccacatt gaagaaagaa tcgaatattc attcatcaaa
401   tcattcactc catagtccga tagttctttt aaagaactga ttaagcagac
451   gagaataaag atagagtccc attctacatg tcaataccga caacaatgaa
501   atttatagta agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details