JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ188973.1
Database:	nt	Go to original record
Description:	Trema domingense voucher SIU:Garwood 5-10 plastid, complete genome.
Taxon:	Trema domingense
Original size:	156617 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49103	49691	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   atggggcaat cctgagccaa atccggtttt ctgaaaacaa acaaggattc
151   agaaagcgat aataaaaaag aataggatag gtgcagagac tcaatggaag
201   ctgttctaac aaatggagtt ggctgcgtta cgttaataaa ggaatccctt
251   ccatcgaaac tccagaaagg atgaagaata aacgtatata cgtacaaaaa
301   tcctatctcc aaataattaa tgaaaatccg aatccgtatt tcttttaatt
351   ttcatgaaaa aggaaagaat tgttgtgaat caattttaag ttgaaaaacg
401   aatggaatat tcattgatca aatcatttac tccatcaaaa tctgatagat
451   cttttgaaga cttgattaat cggatgagaa taaagataga gtcccattct
501   acatgtcaat attgataaga atgaaattta tagtaagagg aaaatccgtc
551   gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details