JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ953913.1
Database:	nt	Go to original record
Description:	Wrightia natalensis voucher S. P. Bester 9357 (PH) plastid, partial genome.
Taxon:	Wrightia natalensis
Original size:	155244 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48429	48999	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atgaaggata aacacatata catacgtatt gaatgaatac tatatcaaat
301   gttaatgccg acccgaatga atctgtattt ttttctataa aaaacggaag
351   aattggtgtg attcgattct ttcttcaatg tggaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgtagatc ttcttttcaa gaactgatta
451   atcggacgag aataaagata gagtcccgtt ctacatgtca atgctggcag
501   caatgaaatt tatagtaaga ggaaaatccg tcgacttaaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details