JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ953906.1
Database:	nt	Go to original record
Description:	Neobracea bahamensis voucher M. Vincent 11466 (GH) plastid, partial genome.
Taxon:	Neobracea bahamensis
Original size:	155693 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48606	49183	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgtagagaa atctttccat cgaaaattca gaaaggatga
251   aggataaaca catatacata cgtattgaat actatatcaa aatcaaatga
301   ttaatgccga cccgaacgaa tccgtatttt ttttctataa aaaacggaag
351   aattggtgtg attcgattct ttcttcaatg tggaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgtagatc ctcttttcaa gaacggatta
451   atcgggcgag aatcgagaat aaagatagag tcccgctcta cgcgtcaatg
501   ctggcaacaa tgaaatttat agtaagagga aaatccgtcg acttaaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details