JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ953903.1
Database:	nt	Go to original record
Description:	Aganosma cymosa voucher D. J. Middleton et al. 1498 (A) plastid, partial genome.
Taxon:	Aganosma cymosa
Original size:	153816 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48156	48716	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgctgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaagg
251   atggaggata tacatatgta ttgaatacta tatcaaatga ttaatgccga
301   cccgaatgaa tctatatttt ttttctataa aaaacggaag aattggtgtg
351   attcgattct ttcttcaatg tggaatcgaa tattcattga tcaaacgatt
401   cactccatag tctgtagatc ctcttttcaa gaacggatta atcggacgag
451   aataaagata gagtcccgtc ctacatgtca atgccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgacttaaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details