JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ920234.1
Database:	nt	Go to original record
Description:	Micraira sp. JLC-2014 plastid, complete genome.
Taxon:	Micraira sp. JLC-2014
Original size:	135318 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46692	47301	+	610 bp

Amplicon:

610 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acttgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga ggtaattacg ttatgttggt agtgaaactc cctctaaatt
251   agagaaagaa gggctttata catctaatac acacgtatag atactgacat
301   agcaaacgat taatcacaga acctatatca taatataggt tctttattca
351   ttttttacaa tgaaattagg aatgattatg aaatagaaaa ttctgaattt
401   ttagaattat tgtgaatcca ttccaatcga atattgagta atcaaatcct
451   tcaattcatt gtttttgaca tcttttaaaa agcggattaa tcggacgagg
501   ataaagagag agtcccattc tacatgtcaa tactgacaac aatgaaattt
551   ctagtaaaag gaaaatccgt cgactttata agtcgtgagg gttcaagtcc
601   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	610	591	2.75e-08

Match details