JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ920226.1
Database:	nt	Go to original record
Description:	Chaetobromus involucratus subsp. dregeanus plastid, complete genome.
Taxon:	Chaetobromus involucratus subsp. dregeanus
Original size:	132725 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	45736	46346	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaagacaagc ggtcctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aggtaattac gttgtgctgg tagtgtaatt ccttctaaat
251   tagagaaaga ggggcttata catctaatac acacgtatag atactgacat
301   aggaaacgat taatcacaga acccatatca taatataggt tctttattta
351   ttttttagaa tgaaattaga aatgaatatg aaatagaaaa ttcagaattt
401   ttttagaatt attgtgaatc tattccaatt gaatattgag taatcaaatc
451   cttcaattca tttttttgag atcttttaaa aagcggatta atcggacgag
501   gataaagaga gagtcccatt ctacatgtca atactgacaa caatgaaatt
551   tctagtaaaa ggaaaatccg tcgactttat aggtcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details