JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ169307.1
Database:	nt	Go to original record
Description:	Trema domingense chloroplast, partial genome.
Taxon:	Trema domingense
Original size:	156671 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49161	49752	+	592 bp

Amplicon:

592 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccccgg aattaaaaaa
101   atggggcaat cctgagccaa atccggtttt ctgaaaacaa acaaggattc
151   agaaagcgat aataaaaaag aataggatag gtgcagagac tcaatggaag
201   ctgttctaac aaatggagtt ggctgcgtta cgttaataaa ggaatccctt
251   ccatcgaaac tccagaaagg atgaagaata aacgtatata cgtacaaaaa
301   tcctatctcc aaataattaa tgaaaatccg aatccgtatt tcttttttta
351   attttcatga aaaaggaaag aattgttgtg aatcaatttt aagttgaaaa
401   acgaatggaa tattcattga tcaaatcatt tactccatca aaatctgata
451   gatcttttga agacttgatt aatcggatga gaataaagat agagtcccat
501   tctacatgtc aatattgata agaatgaaat ttatagtaag aggaaaatcc
551   gtcgacttta aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	592	573	2.67e-08

Match details