JRC GMO-Amplicons | EURL GMFF

Record ID:	MZ905466.1
Database:	nt	Go to original record
Description:	Wenchengia alternifolia isolate VN chloroplast, complete genome.
Taxon:	Wenchengia alternifolia
Original size:	152398 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47950	48521	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacccactaa gtgataactt tcaaattcag agaaaccccg gaattaataa
101   aaatgggcga tcctgatcca aatcctattt tatcaaaaca ttttatcaaa
151   acaaaggttc aaaaaacgaa aaaaaaagga taggtgcaga gactcaatgg
201   aagctgttct aacaaatgga gttgactgcg ttggtagagg aatcctttcc
251   gaggaaactt cagaaaggat gaaggataaa cgtatctatt gaatactata
301   tcaaatgatt aatgatggcc cgaatccgta ttttttaata taaaaaatgg
351   aagaattgat gtgaattgat tccacattga agaaaaaatc gaatattcat
401   ccatcaaatc attcactcca cagtccgata gatcttttaa aggactgatt
451   aattggacga gaataaagat agagtcccat tctacatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details