JRC GMO-Amplicons | EURL GMFF

Record ID:	MW802189.1
Database:	nt	Go to original record
Description:	Wenchengia alternifolia isolate HN chloroplast, complete genome.
Taxon:	Wenchengia alternifolia
Original size:	152961 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48229	48799	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tgatgtggga
51    aacctactaa gtgagaactt tcaaattcag agaaaccccg gaattcataa
101   aaatgggcga tcctgatcca aatcctattt tatcaaaaca ttttatcaaa
151   acaaaggttc aaaaaacgaa aaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tggtagagga atcctttccg
251   aggaaacttc agaaaggatg aaggataaac gtatctattg aatactatat
301   caaatgatta atgatggccc gaatccgtat tttttaatat aaaaaatgga
351   agaattgatg tgaattgatt ccacattgaa gaaaaaatcg aatattcatt
401   catcaaatca ttcactccac agtccgatag atcttttaaa ggactgatta
451   attggacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details