JRC GMO-Amplicons | EURL GMFF

Record ID:	MW559065.1
Database:	nt	Go to original record
Description:	Averrhoa bilimbi voucher J.Wen10211 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Averrhoa bilimbi
Original size:	983 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	574	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acgtactaag tgagaacttt caaattcagc gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaca aagttttaga
151   aagcgagaat aaaaaaggat aggtgcagag actcaacgga agctattcta
201   aaatttacaa atagagttga ctgcgttgcg ttagtaaggg aatccttccc
251   tcaaaattcc agaaaggata aacctatata catacgtata cgtactgaaa
301   tactatctca aatgattaat gatgacctga atctgtatta tttataaaaa
351   taatgaaaga tttcttgtga gtcgattcca agttgaagaa agaatcgaat
401   gataattgat caaatcattc attccagttc ggatagatct tttgaaaacc
451   tgatagaata aagaataaag atagagtccc attctacatg tcaatattga
501   caacaatgaa atttatagta agaggaaaat ccgtcgactt tagaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details