JRC GMO-Amplicons | EURL GMFF

Record ID:	MW699773.1
Database:	nt	Go to original record
Description:	Trikeraia hookeri var. hookeri chloroplast, complete genome.
Taxon:	Trikeraia hookeri var. hookeri
Original size:	137696 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47554	48152	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagc ggttctcgaa
151   ctagaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatcgaat
201   taattacgtt gtgttggtag tggaattcct tctaaattag agagagaggg
251   gctttataca tctaataaac acgtatagat actgacatag caaacgatta
301   atcacagaac ccatatcata atataggttc tttattcttt tttagaatga
351   aattaggaat gattatgaaa taaaaaattc agaatttttt tcgaattatt
401   gtgaattcat tccaatcgaa tattgagtaa tcaaatcctt caattcatag
451   ttttcgagat cttttaaaaa gtggattaat cggacgagga caaagagaga
501   gtcccattct acatgtcaat actggcaaca atgaaatttc tagtaaaagg
551   aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details