Match details

Record ID: MN879738.1
Database: nt Go to original record
Description: Virus synthetic construct clone Tobacco mosaic virus infectious clone, complete sequence.
Taxon: virus synthetic construct
Original size: 11045 bp

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 10523 10605 + 83 bp

Amplicon: 83 bp
1     gcctctaccg acagtggtcc caaagatgga ccccccaccc acgaggaaca
51    tcgtggaaaa agaagacgtt ccaaccacgt ctt 

View other 5 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerB 100.0 22 bp 0 0 83 62 3.59e-10
probe 95.7 23 bp 0 1 21 43 1.67e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.