JRC GMO-Amplicons | EURL GMFF

Record ID:	ON124917.1
Database:	nt	Go to original record
Description:	Mentha longifolia chloroplast, complete genome.
Taxon:	Mentha longifolia
Original size:	152078 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46913	47490	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggtttcaaa
151   aaacgaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgtcgg tagaggaatc tttccatgga aactttattt
251   tataaaggat gaaagataaa cgcatctatt gaatactata tcaaatgatt
301   aatgttggcc cgaatctatt tttttaatat ttaatatgaa aatgaaaaaa
351   tggaaaaatc ggtgtgaatt tttttcacgt tgaagaaaaa atagaatatt
401   catcaactca ttcactccgt agtccgatag atcttttaaa gaacttatta
451   atcggacgag aataaagata gagtcccatt ctacatgcta catatcaata
501   ccggcaacaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details