JRC GMO-Amplicons | EURL GMFF

Record ID:	MW297177.1
Database:	nt	Go to original record
Description:	Kalanchoe sp. DIR059 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Kalanchoe sp. DIR059
Original size:	909 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	577	+	577 bp

Amplicon:

577 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtacggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ttgttttccg aaaccaaacc caaatccatg
151   ggttcaaaaa gcgagaatca aaaaagatag gtgcagagac tcaacggaag
201   ttgttctaac aaatagagtt tactacgtta cattggcaaa ggaatctttt
251   catcgaaact ccagaaagga tgaaggataa atcttaatat acatatgtat
301   acgcactgaa atagtatatc aaatgattaa tgacgcttta ttttcaaatg
351   aaagaattgt taagaagcga ttccgagttg aagaaagaat cgactattca
401   ttgatcaaag aagtgtcact ccacagtctg atagatcttt tgacgaactg
451   agattaatcc gacgagaaaa aagatagagt cccattatac atgtcaatac
501   tgacaacaag gaaattgata gtaaaaggaa aatccgtcga ctttataaat
551   catgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	577	558	2.60e-08

Match details