JRC GMO-Amplicons | EURL GMFF

Record ID:	MW274725.1
Database:	nt	Go to original record
Description:	Cyathopus sikkimensis voucher US:Soreng 5224b trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cyathopus sikkimensis
Original size:	1644 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	685	1304	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaaga gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttttaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgac
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aatgaaattt gaaatagaaa tgattatgaa atcaaaaatt
401   cagaattttt tttagaatta ttgtgaatac attccaatcg aatatttagt
451   aatcaaatcc ttcaattcaa agtttttagg tctttaaaaa agtggattaa
501   tcggacgagg acaaagagag agtcccattc tacatgtcaa tactgacaac
551   aatgaaattt ctagtaaaag gaaaatccgt cgactttata agtcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details