JRC GMO-Amplicons | EURL GMFF

Record ID:	MT648690.1
Database:	nt	Go to original record
Description:	Cerbera manghas chloroplast, complete genome.
Taxon:	Cerbera manghas
Original size:	154461 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47305	47863	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaactcaga gaaaccccgg aattaataaa
101   aagggcaatc ctgagccaaa tcctgttttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaatcttt ccatcgaaaa ttcagaaggg
251   atgaaggata aacgtatata catattgaat actatatcaa atgattaatg
301   ccgacccgaa tgaatccgta ttttttctat aaaaatcgaa gaattggtgt
351   aattcgattc tttcttcaat gtggaatcga atattcattg atcaaatgat
401   ttactccaga gtctgtagat cttttcaaga actgattaat cggacgagaa
451   taaagataga gtcccgttct acatgtcaat gctggcaaca atgaaattta
501   tagtaagagg aaaatccgtc gacttaaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details