JRC GMO-Amplicons | EURL GMFF

Record ID:	OM691673.1
Database:	nt	Go to original record
Description:	Salvia rosmarinus isolate BPTPS048 voucher LISE:96332 chloroplast, complete genome.
Taxon:	Salvia rosmarinus
Original size:	151585 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46408	46975	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgcc ggtagaggaa tctttccatg gaaactttag
251   aaaggatgaa ggataaacgc atctatttaa tactatatca aatgattaat
301   gttgacccga atctgttttt ttaatatgaa aatgagaaaa tggaaaaata
351   ggtgtgaatt tatttcacgt tgaagaataa atcgaatatt catcaactca
401   ttcactccat agtccgatag atcttttaaa gaacttatta atcggacgag
451   aataaagata gagtcccgtt ctacatgcta catgtcaata ccggcagcaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details