JRC GMO-Amplicons | EURL GMFF

Record ID:	OM691672.1
Database:	nt	Go to original record
Description:	Salvia rosmarinus isolate BPTPS028 voucher LISE:96331 chloroplast, complete genome.
Taxon:	Salvia rosmarinus
Original size:	151577 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46408	46977	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttgactgcg ccggtagagg aatctttcca tggaaacttt
251   agaaaggatg aaggataaac gcatctattt aatactatat caaatgatta
301   atgttgaccc gaatctgttt ttttaatatg aaaatgagaa aatggaaaaa
351   taggtgtgaa tttatttcac gttgaagaat aaatcgaata ttcatcaact
401   cattcactcc atagtccgat agatctttta aagaacttat taatcggacg
451   agaataaaga tagagtcccg ttctacatgc tacatgtcaa taccggcagc
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details