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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MZ417371.1
Database: nt Go to original record
Description: Transformation vector pJF1005, complete sequence.
Taxon: Transformation vector pJF1005
Original size: 15585 bp

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3256 3450 + 195 bp
Amplicon: 195 bp 
1     gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat
51    ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
101   cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat
151   gtgacatctc cactgacgta agggatgacg cacaatccca ctatc

View other 71 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 3.10e-08
primerB 100.0 20 bp 0 0 195 176 9.28e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.