JRC GMO-Amplicons | EURL GMFF

Record ID:	AF115481.1
Database:	nt	Go to original record
Description:	Prunus salicina tRNA-Leu (trnL) gene, chloroplast gene encoding chloroplast RNA, complete sequence.
Taxon:	Prunus salicina
Original size:	598 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	597	+	587 bp

Amplicon:

587 bp

1     cgaaatcggt agacgctacg gacttctata agcaccgcgc tacgctgaaa
51    cggtaccgct acgcgagaac tttcaaatcc acagaaaccc tggaattaaa
101   aatgggcgat cctgagccaa atcctgtttt attaaaacaa acaagggttt
151   cataaaccga gaataaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttggct gcattgtgtt agtaaaggaa tccttacatc
251   gaaacttccg aaaggatgaa ggataaacct atatgcatac gtatagtact
301   gcaatactat ctccaaatga ttaatgacgg ctcgaatctg tattttttta
351   tatttatatg aaaaacgaaa gaattgttgt gaatcgatta aaaattgaaa
401   aaagaatcga atattcattg atcaaatcat tcactcatca tagtcctgtc
451   gatcttttta agaattgttt tatcggacga gaataaagat agagtccatt
501   atacatgcaa tatcgcacat gaaatttata gtatgaggaa aatccgtcga
551   ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	587	568	2.64e-08

Match details