JRC GMO-Amplicons | EURL GMFF

Record ID:	KJ729813.1
Database:	nt	Go to original record
Description:	Tamarix ramosissima tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tamarix ramosissima
Original size:	1047 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	601	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggcatgctg
51    actagtaggg aacctactaa gtgataactt tcaaattcag aggaaaccct
101   ggaaattaat aaaaaatggg cacatctctg agccaaactc ccgtttttcc
151   aaaaggtaaa aaaaaaaaaa aattattaaa gcgagaataa ataatggata
201   ggtgcagaga ctcaacggaa gctgttctaa caaatggagt ttactgtatt
251   attataagaa ttcttccata aaaactgcaa aaaaaagatg aagaagaacc
301   ctatatctag ataaatacgt actaaaatat tctcaaaaat aaaaaaacgt
351   attaatgacg ggcaaatatg tcttttttac cttttgtcta ggaaaaaatg
401   taagaatatt aaattatcaa agtattcact ccatggtctg atagatcctt
451   ttttttgtac gaactgctca atcggacgag aataaagata gagtcccatt
501   ctacatgtca atactgacaa caatgaaatt tatagtatga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details